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HIV endocytosis after dendritic cell to T cell viral transfer leads to productive virus infection.

01/01/2009

Antiviral Res 83, 94-98.

AbstractContacts between HIV-producing T cells and primary CD4+ T cells may induce the uptake of HIV by target cells that are endocytosed into trypsin-resistant compartments.We have now compared the mechanism of virus transmission from T cell-to-T cell versus infected dendritic cells (DCs)-to-T cell. In cocultures of HIV-1-infected DCs with primary CD4+ T cells, virus transmission to the target cells was resistant to trypsin treatment and could only be prevented by the anti-SUgp120 antibody IgGb12 but not by TAK-779, C34 or AZT. Importantly, upon stimulation of purified HIV-1-loaded CD4+ T cells with PHA/IL-2, cells became productively infected as measured by intracellular CAp24 staining and antigen determination in the cell supernatant. These results suggest that the viral endocytic transfer may represent a escape mechanism in the presence of drugs targeting HIV-1 entry or the host immune system.
Clotet-Codina, I., Bosch, B., Senserrich, J., Fernandez-Figueras, M. T., Pena, R., Ballana, E., Bofill, M., Clotet, B., and Este, J. A.

Grups vinculats: Entrada Viral i Patogènesi

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